Perform direct functions
July 29, 2013, 715003794 Rev. B 131
4. Select Wet Prime, and then press Enter.
5. Enter a flow rate of 0.000 mL/min and a time of 5 min.
After the solvents in the vacuum degasser are equilibrated and the
separations module is prepared to run, perform any of the following functions
as necessary:
• Purge the injector
• Purge the refractometer reference cell
• Condition the column
• Equilibrate the system
Purge the injector
Purge the injector for these reasons:
• To remove any traces of solvent from a previous run
• To clear air bubbles from the flow path or the syringe
• To switch to a new (miscible) solvent after you wet prime
The purge process can include a compression check to test the needle seals.
To purge the injector:
1. Press the Direct Function screen key in the Status screen.
2. Select the Purge Injector option, and then press Enter.
3. In the Purge Injector dialog box, specify 6 sample loop volumes.
Tip: To perform a compression test after the purge, move the cursor to
the Compression check box and press any numeric key.
4. Press OK.
Caution: To avoid precipitating salts in the separations module, use an
intermediate solvent such as water when you change from buffers to
solvents of high organic content. See “Solvent miscibility” on page 297
for information on solvent miscibility.
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