3 Prepare the e2695 Separations Module for Operation
114 July 29, 2013, 715003794 Rev. B
Change from a buffered solvent to an organic solvent
To change from a buffered solvent to a totally organic solvent in the
separations module:
1. Remove the solvent line from the reservoir containing the buffered
solvent.
2. Specifying a flow rate of 2.000 mL/min, perform a wet prime until air
begins to enter the line (see “Wet prime the system” on page 129).
3. Place the previously removed solvent line into filtered HPLC-grade
water, and perform a wet prime for 0.5 min at 5 mL/min (see “Wet prime
the system” on page 129).
4.
Equilibrate the solvent in the degasser chamber by setting a flow rate of
0.000 mL/min for 5 min
(see “Equilibrate solvents in the in-line vacuum
degasser” on page 130).
5. Purge the injector for a minimum of 6 loop volumes (see “Purge the
injector” on page 131).
6. Remove the solvent line from the HPLC-grade water.
7. Fill a clean solvent reservoir with the organic solvent and place the
solvent line in the reservoir.
8. Perform the procedure, above, for preparing an idle or powered-off
separations module for operation.
Power-off the separations module
Before you power-off the separations module, remove any buffered mobile
phase present in the flow path (see “Flushing the flow path” below).
Caution: To avoid salts from precipitating in the separations module,
use an intermediate solvent such as water when you change from
buffers to high-organic-content solvents (see “Solvent miscibility” on
page 297).
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