D Solvent Considerations
292 July 29, 2013, 715003794 Rev. B
Solvent quality
Use HPLC-grade solvents to ensure the best possible results. Filter solvents
through 0.45-µm filters before use. Solvents distilled in glass generally
maintain their purity from lot to lot; use them to ensure the best possible
results.
Solvent reservoirs
The following recommendations apply to the solvent reservoirs used with the
separations module:
• When using the instrument for general chromatography (i.e. reverse
and/or normal phase and gel permeation [GPC]), Waters recommends
the use of high-quality lab glassware made of borosilicate glass for all
reservoirs (solvent, seal wash & needle wash).
• When using techniques such as ion chromatography, where glass
containers can contribute ionic contamination (sodium and/or chloride
ions), it is acceptable to use laboratory-grade polypropylene or
polyethylene containers as reservoirs.
• When using the instrument in combination with a mass spectrometric
detector, please refer to recommendations within the most recent
version of “Controlling Contamination in UPLC/MS and HPLC/MS
Systems” at the Waters’ website (www.waters.com) in the Support
Library under Waters Division/Services & Support.
Preparation checklist
The following solvent preparation guidelines help to ensure stable baselines
and good resolution:
• Filter solvents with a 0.45-µm filter.
• Degas the solvent.
• Stir the solvent.
• Keep solvents in a location that is free from drafts and shock.
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