Procedures for preparing the separations module for operation
July 29, 2013, 715003794 Rev. B 113
Tip: To maximize degasser efficiency (and fill all four degasser chambers
with solvent), fill all four solvent reservoirs (even if you use fewer than
four solvents). If you choose water as one of the “unused” solvents,
change the water weekly to prevent bacterial contamination.
4. Set the degasser to On (see “Equilibrate solvents in the in-line vacuum
degasser” on page 130).
5. Perform a dry prime to fill each solvent line and degasser chamber (see
“Dry prime the system” on page 126).
6. Equilibrate the solvent in the degasser chamber by setting a flow rate of
0.000 mL/min for 5 min (see “Equilibrate solvents in the in-line vacuum
degasser” on page 130).
7. Run System Prep (see “Run System Prep” on page 128).
Prepare an idle or powered-off separations module for operation
To prime, equilibrate, and purge the separations module that is powered-off
or idle with no solvent flow:
1. Check plunger seal-wash fluid levels. Refill and prime, if necessary (see
“Prime the plunger seal-wash pump” on page 106).
2. Check needle-wash fluid levels. Refill and prime, if necessary (see
“Prime the needle-wash pump” on page 109).
3. Set the degasser to On (see “Equilibrate solvents in the in-line vacuum
degasser” on page 130).
4. Run System Prep (see “Run System Prep” on page 128).
Caution: If you just performed the steps from the procedure, below, for
changing from a buffered solvent to an organic solvent, ensure that the
Plunger Seal-Wash and Needle-Wash solvents are compatible with the
new solvents.
To Next Page
Loading...
