4 Front Panel Control
130 July 29, 2013, 715003794 Rev. B
To wet prime the system:
1. In the Status screen’s Composition field, enter 100% for the solvent you
want to use for wet priming.
Tip: To facilitate purging air from the lines, start the wet prime using
the solvent with the lowest viscosity.
2. On the Status screen, press the Direct Function screen key.
3. Select Wet Prime, and then press Enter.
4. Gently shake the filters in the reservoirs to remove any bubbles.
5. In the Wet Prime dialog box, specify the flow rate and duration of the
priming (using the composition entered in the Status screen), and then
press the OK screen key.
Result: The solvent management system begins to operate at the end of
the specified time period, the solvent management system returns to the
previous conditions, and the separations module enters the idle mode.
6. Repeat step 1 through step 5 for each solvent, as appropriate.
Equilibrate solvents in the in-line vacuum degasser
To equilibrate solvents in the vacuum degasser after you wet primed the
system:
1. In the Status screen, specify the initial solvent composition for the run.
2. Set the degasser mode parameter to ON.
3. Press the Direct Function screen key.
Caution:
• To avoid damaging the plunger seals, perform a wet prime only when
solvent is in the solvent management system flow path. If necessary,
fill all solvent reservoirs and dry prime the system before you wet
prime.
• To avoid precipitating salts in the separations module, use an
intermediate solvent such as water when you change from buffers to
solvents of high organic content. See “Solvent miscibility” on
page 297 for information on solvent miscibility.
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