3 Prepare the e2695 Separations Module for Operation
112 July 29, 2013, 715003794 Rev. B
5. Close the carousel door.
If you enable the “Verify carousel placement” check box (the default setting) in
the Configuration screen, an error message appears if you attempt to make an
injection from a carousel that is in the wrong position.
After the carousel is engaged, all subsequent carousel movements are
monitored using a sensor. If the sensor detects that a carousel movement has
failed, the movement is retried. If it fails a second time, a “Carousel
malfunction” error is generated. This ensures that the carousel is always
positioned correctly.
Procedures for preparing the separations module for
operation
This section explains how to prepare the separations module. The instructions
provide the sequence of steps to prime, equilibrate, and purge a separations
module that is in one of the following states:
• A new or dry separations module.
• A separations module that is powered-off or left idle with no flow.
• A separations module that requires a solvent change (from a buffered
solvent to an organic solvent).
For step-by-step instructions on how to use direct functions to prime,
equilibrate, and purge your separations module, and to fully ready the
separations module for running samples, see “Perform direct functions” on
page 123.
Prepare a new or dry separations module for operation
To change from a buffered solvent to a totally organic solvent:
1. Prime the plunger seal-wash pump (see “Prime the plunger seal-wash
pump” on page 106).
2. Prime the needle-wash pump (see “Prime the needle-wash pump” on
page 109).
3. Fill all four solvent reservoirs with fresh solvent filtered and degassed
by vacuum filtration (see “Prepare solvent reservoirs” on page 104).
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