Power-off the separations module
July 29, 2013, 715003794 Rev. B 115
Flush the flow path
To flush buffered mobile phase from the flow path:
1. Replace the buffered mobile phase with HPLC-quality water, and wet
prime the system for 10 minutes at 3 mL/min (see “Wet prime the
system” on page 129).
2. Perform three injector purge cycles (see “Purge the injector” on page
131).
Requirement: The sample loop must be clean.
3. Replace the plunger seal-wash solvent with a fresh solution of 80%
water/20% methanol or a miscible solvent, and prime the plunger
seal-wash pump (see “Prime the plunger seal-wash pump” on page 106).
4. Replace the water mobile phase with a solution of 90% methanol/10%
water, and flush the system for 10 minutes at 3 mL/min.
5. Replace the needle-wash solvent with a solution of 90% methanol/10%
water or a miscible solvent, and perform a needle-wash prime (see
“Prime the needle-wash pump” on page 109).
Power-off the separations module
To power-off the separations module:
1. Ensure that the system is flushed properly (see “Flush the flow path” on
page 115).
2. Press the power switch to the (Off) 0 position.
Tip: The time and date are entered in the log file.
Caution: To avoid damaging your column, remove the column before you
perform the following procedure.
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