Introduction
July 29, 2013, 715003794 Rev. B 293
Water
Use water only from a high-quality water purification system. If the water
system does not provide filtered water, filter it through a 0.45-µm membrane
filter before use.
Buffers
When you use buffers, dissolve salts first, adjust the pH, then filter to remove
insoluble material.
Tetrahydrofuran (THF)
When using unstabilized THF, ensure that your solvent is fresh. Previously
opened bottles of THF contain peroxide contaminants, which cause baseline
drift.
GPC solvents and seal choices
For GPC separations, see the following table for plunger seal and seal wash
solution recommendations. If required, use an intermediate solution to
prevent immiscibility or precipitation problems when you switch between
GPC and reversed-phase analytical solvents.
Warning: THF contaminants (peroxides) are potentially explosive if
concentrated or taken to dryness.
Recommended plunger seal and seal wash solution to be used for GPC
solvents:
GPC Solvent
With standard
seal
a
Fluoroloy G
(yellow)
With optional
seal
b
Alliance Clear
Seal
(opaque)
Seal wash
solution
Chloroform Recommended Recommended 100% methanol
Dimethylsulfoxide
(DMSO)
Recommended Recommended 100% methanol
To Next Page
Loading...
