4 Front Panel Control
128 July 29, 2013, 715003794 Rev. B
Result: The solvent management system begins to operate. At the end of
the priming period, the solvent management system turns off and the
separations module enters the idle mode.
13. Perform a wet prime.
Run System Prep
The System Prep function performs a series of automated steps, putting the
separations module in a condition of complete system readiness for running
samples. System Prep is useful when you are changing solvents or after
extended periods of system inactivity.
System Prep initiates the following sequence of functions:
• The specified flow rate, composition, sample temperature, and column
temperature settings are applied.
• The duty cycle of the seal-wash pump is set to 1 second.
• Simultaneously, the seal-wash pump and needle-wash pump operate for
40 seconds (“Extended” wash time).
• Sequentially, each selected solvent line is wet primed at 7.0 mL/min for
0.5 minutes.
• The specified flow rate, composition, sample temperature, and column
temperature settings are applied.
• The system pauses for 30 seconds to condition the solvent management
system.
• The Purge Injector function runs with its default parameters (see “Purge
the injector” on page 131).
• The specified flow rate, composition, sample temperature, and column
temperature settings are applied.
The System Prep function is a simple to use alternative to running the
individual functions of Wet Prime, Purge Injector, Equilibrate, and Condition
Column, all of which are described later in this chapter. Additionally, using
System Prep, you can specify the separation method parameters stored for
when you run System Prep again.
Tip: You can access the System Prep function from either the Main screen or
the Direct Functions list. You can also run it from a sample set that is stored
in the separations module. When you do so, System Prep adopts the
separation method’s parameter values.
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