Waters Alliance e2695 User Manual

D Solvent Considerations
304 July 29, 2013, 715003794 Rev. B
Mixed mobile phases
The following table provides approximate wavelength cutoffs for some other
solvents, buffers, detergents, and mobile phases. The solvent concentrations
represented are those most commonly used. If you want to use a different
concentration, you can determine approximate absorbance using Beer’s Law,
since absorbance is proportional to concentration.
Wavelength cutoffs for different mobile phases:
Mobile phase
UV
Cutoff
(nm)
Mobile phase
UV
Cutoff
(nm)
Acetic acid, 1% 230 Sodium chloride, 1 M 207
Ammonium acetate, 10 mM 205 Sodium citrate, 10 mM 225
Ammonium bicarbonate,
10 mM
190 Sodium dodecyl sulfate 190
BRIJ 35, 0.1% 190 Sodium formate, 10 mM 200
CHAPS, 0.1% 215 Triethyl amine, 1% 235
Diammonium phosphate, 50
mM
205 Trifluoracetic acid, 0.1% 190
EDTA, disodium, 1 mM 190 TRIS HCl, 20 mM, pH 7.0,
pH 8.0
202,
212
HEPES, 10 mM, pH 7.6 225
Triton-X
™
100, 0.1%
240
Hydrochloric acid, 0.1% 190 Waters PIC Reagent A,
1 vial/liter
200
MES, 10 mM, pH 6.0 215 Waters PIC Reagent B-6,
1 vial/liter
225
Potassium phosphate,
monobasic, 10 mM
dibasic, 10 mM
190
190
Waters PIC Reagent B-6,
low UV, 1 vial/liter
190
Sodium acetate, 10 mM 205 Waters PIC Reagent D-4,
1 vial/liter
190

Waters Alliance e2695 Specifications

General

Type	HPLC System
Flow Rate Range	0.001 to 10.0 mL/min
Gradient Formation	Quaternary gradient
Number of Solvents	4
Injection Volume Range	0.1 to 100 μL
Sample Capacity	120 vials
Sample Temperature Control	4°C to 40°C
Communication Interface	Ethernet
Power Requirements	100–240 VAC, 50/60 Hz