Perform direct functions
July 29, 2013, 715003794 Rev. B 133
Condition the column
You condition the column by running a solvent gradient through it without
injecting samples or running the events table. You select a separation method
(see Chapter 6) that contains the gradient parameters, and the separations
module runs the gradient method.
Tip: To run timed events without injecting a sample, select the Inject Samples
option from the Direct Functions menu and enter an injection volume of 0 (see
“Inject samples” on page 133).
To condition the column:
1. Press Menu/Status to display the Status screen.
2. In the Status screen, select the Method field, and then select the
separation method with which you want to condition the column.
3. Press the Direct Function screen key.
4. Select Condition Column, and then press Enter.
5. In the Condition Column dialog box, specify a length of time for
conditioning the column that equals or exceeds the duration of the
gradient, as defined in the specified separation method (including
reequilibration time).
6. Press the OK screen key.
Inject samples
Using the keypad, you can inject one or more samples from one or more
contiguous vials. The separations module operates according to the
instrument conditions displayed in the Status screen.
If you make any changes to the conditions displayed in the Status screen, the
Method field indicates “<direct>,” the separation method name in the Banner
area appears within brackets (< >), and the current isocratic conditions (with
no timed events) are used for the injection. Choosing a separation method
removes the “<direct>” label from the method field and uses any programmed
gradients or timed events.
To inject a sample:
1. Place the prepared sample vial(s) in a carousel.
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