July 29, 2013, 715003794 Rev. B xv
Monitor your HPLC system ........................................................................... 119
Perform direct functions ................................................................................ 123
Dry prime the system ...................................................................................... 126
Run System Prep ............................................................................................. 128
Wet prime the system...................................................................................... 129
Purge the 2410 and 410 reference cell............................................................ 132
Equilibrate the system .................................................................................... 132
Condition the column....................................................................................... 133
Inject samples .................................................................................................. 133
5 Automatic Runs ..................................................................................... 135
System Controller or No Interaction mode..................................................... 135
Controlled by Empower software.................................................................... 136
Controlled by MassLynx software .................................................................. 136
Perform automatic runs in a standalone mode ........................................ 136
Run a sample set.............................................................................................. 137
View sample sets.............................................................................................. 139
Perform a run from a sample template .......................................................... 140
Modify a sample set during a run................................................................... 141
Stop a run......................................................................................................... 142
Perform automatic runs under Empower software control .................. 142
Perform automatic runs under MassLynx control .................................. 144
Perform an automatic run using MassLynx software ................................... 144
6 Create Methods, Sample Sets, and Sample Templates ................. 145
Separation methods ......................................................................................... 145
Sample sets ...................................................................................................... 146
Sample templates ............................................................................................ 146
Create and edit separation methods ........................................................... 146
Create a separation method ............................................................................ 148
Edit a separation method ................................................................................ 149
Copy and edit a separation method ................................................................ 149
Lock and unlock a separation method............................................................ 150