8 Diagnostic Testing and Troubleshooting
246 July 29, 2013, 715003794 Rev. B
Chromatography troubleshooting:
Symptom Possible cause Corrective action
Erratic retention
times
Air bubble in plunger
head
Degas all solvents, prime
solvent management system.
Evaluate the degasser
performance. Set the bubble
detect alarm in the Mobile
Phase screen (see “Set Mobile
Phase screen parameter
values” on page 151).
Malfunctioning check
valves
Clean/replace inlet check valve
cartridges (see “Replace an
inlet check valve cartridge” on
page 194).
Leaking plunger seals Replace seals (see “Notes on
replacing the plunger seals” on
page 187).
Separation chemistry Check mobile phase, column,
GPV.
Clogged solvent filters Replace filters.
Increased
retention times
Incorrect flow rate Change flow rate.
Incorrect solvent
composition
Change solvent composition,
check GPV.
Column heater (or
column heater/cooler)
not turned on;
temperature low
Turn column heater (or
column heater/cooler) on,
determine temperature.
Incorrect mobile phase Use correct mobile phase.
Column contaminated Clean/replace column.
Incorrect column Use correct column.
Leak in solvent
management system,
loss of solvent
Inspect fittings for leaks.
Perform Static Leak Test (see
“Static leak test” on page 234).
To Next Page
Loading...
