Troubleshoot
July 29, 2013, 715003794 Rev. B 247
Decreased
retention times
Incorrect flow rate Change flow rate.
Incorrect solvent
composition
Change composition, evaluate
GPV performance.
High column
temperature
Reduce column temperature.
Incorrect mobile phase Replace with correct mobile
phase.
Column contaminated Clean/replace column.
Incorrect column Use correct column.
Solvent not properly
degassed
Degas solvent.
Reproducibility
errors
Solvent not properly
degassed
Degas solvent.
Incorrect
chemistry/integration
Evaluate
chemistry/integration.
Sample management
system problem
Troubleshoot sample
management system.
Baseline drift,
rapid
Column not equilibrated Equilibrate column.
Detector not allowed to
warm up
Allow detector to warm up
until baseline is stable. Time
varies with wavelength and
sensitivity.
Solvent contaminated Use fresh solvent.
Solvent not properly
degassed (rapid or slow
drift)
Degas solvent.
Flow fluctuations (rapid
or slow drift)
Prime solvent management
system, replace pump seals,
check valves (see “Erratic
Retention Times” symptom).
Incorrect wavelength for
solvent
Check that solvent does not
have absorbence at the
wavelength used.
Chromatography troubleshooting: (Continued)
Symptom Possible cause Corrective action
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