8 Diagnostic Testing and Troubleshooting
250 July 29, 2013, 715003794 Rev. B
Carryover is seen
from previous
injection
Previous injection is
exceedingly high in
concentration
Calculate mass injected. Try
reinjecting a larger volume of
less concentrated solution
while keeping injected mass
constant.
Choice of needle-wash
solvent is insufficient to
solubilize all sample
components.
Choose a needle-wash solvent
that solubilizes all
components.
Lower needle-wash frit
is contaminated
Replace lower needle-wash frit
Flat baseline, no
peaks
No solvent flow Determine flow rate.
Lamp not ignited Use detector diagnostics to
determine reference/sample
energy. Zero energy indicates
lamp not ignited.
Ignite lamp. If this does not
resolve the problem, replace
the lamp.
Detector not zeroed Zero the detector baseline.
Improper connection
between detector and
recorder
Examine cabling between the
detector and the recorder.
Incorrect wavelength Ensure correct wavelength
setting.
Leak in solvent path Inspect fittings.
Chromatography troubleshooting: (Continued)
Symptom Possible cause Corrective action
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