Troubleshoot
July 29, 2013, 715003794 Rev. B 251
Flat-topped peaks Detector not zeroed Zero the detector baseline.
Incorrect recorder input
voltage
Adjust recorder input voltage,
or adjust detector output cable
to correct position.
Sensitivity too high Select a less sensitive
detection range.
Sample concentration or
injection volume exceeds
voltage output of
detector
Decrease sample
concentration or injection
volume.
Sensitivity loss Leak in sample
management system
Troubleshoot sample
management system.
Degraded,
contaminated, or
improperly prepared
sample
Use fresh sample.
Column contaminated Clean/replace column.
Loss of column
efficiency
Clean/replace column.
Peaks wider than
expected
Troubleshoot sample
management system. Inspect
fittings for leaks and proper
ferrule setting. Confirm tubing
inner diameter is correct.
Incorrect Filter
Constant
Set correct filter constant on
detector.
Change in mobile phase
composition
Correct mobile phase pH or
ionic composition.
Leak in flow cell Tighten cell seal.
Incorrect flow rate Change flow rate.
Chromatography troubleshooting: (Continued)
Symptom Possible cause Corrective action
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