Calibration Theory
AEROSET
®
Customer Training Guide 6-7
94858-103 — July 2003
Abs
Results are obtained from the absorbance data based on comparison to
the absorbance of water. End-point assays are represented as absorbance,
and rate assays are expressed as rate of absorbance change/minute.
During calibration, sample blank correction is not performed, even when
sample blank is specified for the end-point assay.
Factor
These assays are typically measuring for enzyme activity. Enzyme assays
are measuring a chromophore (NADH, PNP, etc.) that has a specific
response that varies little between different instruments and reagent lots;
therefore, a single factor is used to calculate all results. Only a reagent
blank is performed for calibration. To calculate results, the reagent blank
absorbance is subtracted from the measured absorbance, and the
difference is multiplied by the factor.
Conc = (measured Abs - Rgt Blank Abs) x Factor
NOTE: The single factor used for the Factor method is entered
in the Calibration page of the Assay Configuration screen. For
further information, refer to Module 8, Configuration.
1-Point Linear
A reagent blank and a single calibrator are measured. A calibration curve
is generated using these two data points.
• An example is Sample Interference
Indices, Drugs of Abuse
• All enzymes = rates
• An example is HDL
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