Calibration Theory
AEROSET
®
Customer Training Guide 6-9
94858-103 — July 2003
Spline
The Spline method is a non-linear curve calculation used for data points
that lie on a slight curve. On the AEROSET System, the spline
calibration uses a reagent blank (water) and five levels of calibrators.
Use Fac/Blk (Factor and Blank)
If an assay is defined as Use Fac/Blk, it does not generate a calibration
curve but uses the factors and reagent blank from a calibration curve for
another assay. This method is used when two or more assays use the
same reagents (example: Serum Calcium and Urine Calcium, etc.).
•Show IgA.
• Is a point to point, but cannot easily
calculate factors.
• Curve is basically symmetrical.
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