Applied Biosystems QuantStudio 6 Flex - Page 186

In the thermal prole, a stage that is repeated. A cycling stage is also called an
amplication stage.
See also “amplication stage“ on page 184.
During the instrument run, a process in which an instrument detects uorescence
data from each w
ell of the plate or array card. The instrument transforms the signal to
electronic data and saves the data in the experiment le.
See “baseline-corrected normalized reporter (ΔRn)“ on page 185.
The default view of the melting curve. It plots the negative derivative data (–R
n
′ ) vs.
temperature.
Note: An alternative view is the raw data view, which plots the normalized
uorescence from reporter dye vs. temperature.
The rate of change in uorescence as a function of temperature. R
n
′ is used to plot a
melting curve. Signicant decrease of the uorescent signal generates a positive peak
on the derivative view of the melting curve.
See “melt curve“ on page 187.
Type of calibration in which the software collects spectral data from a series of dye
standards and stores the spectral information for the dye standards in a pure spectra
calibration le. This le is used during experiment runs to characterize and
distinguish the individual contribution of each dye in the total uorescence collected
by the instrument.
See “amplication eciency (EFF%)“ on page 184.
The standard error of the slope of the regression line in the standard curve.
The error can be used to calculate a condence interval (CI) for the slope. Because the
amplication eciency is calculated from the slope, knowing the error allows a CI for
the amplication eciency to be calculated.
Refers to the entire process of performing a run, including setup, run, and analysis.
Dye excitation and emission lter combination that is set for a experiment. The
QuantStudio
™
7 Flex System includes a decoupled, six by six-color lter set. The
QuantStudio
™
6 Flex System includes a coupled ve-color lter set. The excitation and
emission lter combinations are preset by the experiment document (.eds) le for
your experiment.
A quality control (QC) indicator which, when applied by the software to a well during
analysis, indicates a possible issue with that reaction. For example, a ag may be
issued if no amplication is detected in a well. Flags indicating potential problems are
displayed in the Quality Control tab of the Plate Layout, Well Table, and QC
Summary screens.
cycling stage
data collection
delta R
n
(ΔR
n
)
derivative melt
curv
e plot
derivative reporter
(–R
n
′ )
dissociation curve
dye calibration
EFF%
error
experiment
filter
flag
Limited product warranty
186
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide