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Agilent Technologies 1220 Infinity II LC

Agilent Technologies 1220 Infinity II LC
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50 1220 Infinity II LC System User Manual
3
Installation
Performing a ‘Checkout Run’
Performing a ‘Checkout Run’
1 Start the Chromatographic Data System
2 Create a checkout method with the following parameters:
Flow: 1 mL/min
Inj. Volume: 20 μL (Autosampler)
Oven temp: not controlled
VWD Wavelength: 254 nm
Runtime: 1 min
This checkout run is done with the factory installed ‘restriction capillary’ in
place.
3 Prepare 1ml of a checkout sample (acetone for example) and put it on vial
position 1 in the autosampler tray.
For Manual Injector configurations load 20 μL of checkout sample in the
loop. Overfill the injection loop at least 3 times. (e.g. inject at least 60 μL at
a 20 μL sample loop)
4 Start a single run.
As result a single peak should be visible.
5 Print out the report.
6 Store all created and printed out reports in a binder.
The instrument is installed.

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