96 1220 Infinity II LC System User Manual
5
Solvent Delivery System Description
Using the Pump
Using the Pump
Hints for successful use of the Agilent 1220 Infinity II LC pump
• When using salt solutions and organic solvents in the Agilent 1220 Infinity
II LC pump, it is recommended to connect the salt solution to one of the
lower gradient valve ports and the organic solvent to one of the upper
gradient valve port. It is best to have the organic channel directly above the
salt solution channel. Regular flushing with water of all DCGV channels is
recommended to remove all possible salt deposits in the valve ports.
• Before operating the pump, flush the vacuum degasser (optional) with at
least two volumes (3 mL), especially when the pump has been turned off for
some time (for example, overnight) and volatile solvent mixtures are used
in the channels.
• Prevent blocking of solvent inlet filters (never use the pump without solvent
inlet filter). Growth of algae should be avoided.
• Check the purge valve frit and column frit regularly. A blocked purge valve
frit can be identified by black or yellow layers on its surface, or by a
pressure greater than 10 bar when pumping distilled water at a rate of
5 mL/min with an open purge valve.
• When using the pump at low flow rates (for example, 0.2 mL/min), check all
1/16-inch fittings for any signs of leaks.
• When exchanging the pump seals, also exchange the purge valve frit.
• When using buffer solutions, flush the system with water before switching
it off.
• Check the pump plungers for scratches when changing the plunger seals.
Scratched plungers will lead to micro leaks and will decrease the lifetime of
the seal.
• After changing the plunger seals, pressurize the system according to the
wear-in procedure.
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