44 Agilent 8453 UV-visible Spectroscopy System Operator’s Manual
3 Good Measurement Practices
General Considerations
Flow Cells
We recommend a sipper system with a flow cell for obtaining the high
precision measurements. Using a flow cell eliminates the necessity of moving
the cell between blank measurement and sample measurement. Also, the cell
can be rinsed thoroughly with the solution to be measured.
The design of the flow cell should minimize entrapment of bubbles and flow
channeling to provide the most reliable results.
Figure 6 Cells You Should Not Use With the Instrument
Quartz cells with transparent apertures,
fluorescence cells, plastic cells
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