27
Agilent ChemStation for UV-visible Spectroscopy
Operator’s Manual for Biochemical Analysis Software
Agilent Technologies
3
Thermal Denaturation Mode
Preparing a Thermal Denaturation Measurement 28
The Thermal Denaturation Experiment 36
This chapter describes the essential tools to develop a thermal denaturation
method, to acquire and evaluate the data with the thermal denaturation mode
of the biochemical analysis software. The first section, “Preparing a Thermal
Denaturation Measurement" on page 28, contains information about the steps
before the measurement. You will learn how to setup the spectrophotometer,
the temperature ramp for the Peltier Temperature controller and to choose
between different analytical evaluation options.
The second section, “The Thermal Denaturation Experiment" on page 36,
describes the actual thermal measurement, the real-time online monitor and
the possibilities for data processing with the Agilent ChemStation software.
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