Understanding Your Agilent ChemStation 137
Peak Identification
6
Peak Qualifiers
the ratio between the main peak qualifier Lambda
1
and the qualifier peak, for
example, Lambda
3
is within the allowed limits then the compound identity can
be confirmed.
Signal Correlation
Signal correlation means that two peaks measured in different detector
signals within a defined time window are assigned to the same compound. The
signal correlation window can be controlled by the SignalCorrWin parameter in
the QuantParm table of the _DaMethod register. Signal correlation is disabled
when setting the signal correlation window to 0.0 minutes (see the Macro
Programming Guide for more information). When signal correlation is off,
peaks eluting at the same retention/migration time in different detector
signals are treated as different compounds.
The default signal correlation window for LC, CE, CE/MS and LC/MS data is
0.03 minutes and 0.0 minutes for GC data.
Qualifier Verification
If signal correlation is enabled, qualifier verification is active for all data file
types by default. It can be disabled by setting the UseQualifiers flag in the
Quantification Parameters table of the method (see the macro programming
guide for more details). Qualifier verification is also disabled when signal
correlation is switched off.
Qualifier Ratio Calculation
When qualifiers verification is enabled for a compound, the ratio of the
qualifier size and the main peak size is verified against the calibrated limits.
The size may be height or area according to the calculation base setting in
Specify Report.
The qualifier peaks can be calibrated in the same way as the target
compounds. The user does not need to specify the expected qualifier ratio. The
expected qualifier ratio is calculated automatically:
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