Understanding Your Agilent ChemStation 173
Automation
8
What Happens When a Sequence is Run?
What Happens When a Sequence is Run?
Starting a Sequence using “Unique Folder Creation ON”
The system creates a sequence data container based on the path definition in
the sequence parameters and the sequence preference settings. The sequence
template *.s, all methods defined in the sequence table belonging to this
particular sequence are copied to the sequence data container. The system
continue to work with these files during acquisition. Starting the sequence,
the method of the corresponding sequence line is loaded within ChemStation
from out these data container.
Starting a Sequence using “Unique Folder Creation OFF”
Starting a sequence, the system is loading the sequence *.s file and based, on
the entry in the sequence table the corresponding method of the sequence line
is loaded within ChemStation. In contract to the second data storage mode
“Unique Folder Creation ON”, no sequence data container is created. Sequence
and methods will remain in their master directory.
Further executed steps during sequence execution:
The following steps will be repeated for each executed sequence line:
• If equipped with an autosampler, the ChemStation software first locates the
sample in the autosampler according to the number entered in the vial
column
• The instrument is loaded with the method parameters.
• The pre-run macro is executed.
• The sample is then injected into the instrument (manually or
automatically).
• The method data evaluation is done. Integration, quantification, and
reporting, including any user specified macro. In case the mode “Unique
Folder Creation ON” is used, the system is storing two additional methods,
ACQ.M and DA.M during the run.
• The post-run macro is executed.
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