Biochemical Analysis Software for Agilent ChemStation 23
Kinetics Mode 2
The Kinetic Measurement
Running a Time Based Measurement
Measurements can be started either by using the Measure menu from the
menu bar, by pushing the buttons (Blank, Sample, Time Based Measurement)
on the SideBar of the graphical user interface or by using selected F keys.
If you have selected the option Adjust gains separately from blank
measurement in the Options & Info… dialog box, Set Gains is included in the
Measure menu. The separate gain adjustment on a blank medium with high
transmission (for example, water or buffer) sets the gains to a lower value and
avoids an A/D converter overflow. This helps to avoid invalid data points due
to negative slope time traces.
1 Introduce cell filled with water or any other low absorbing medium into the
cell holder.
2 Move multicell transport to the right position.
3 Click on Set gains in the Measure menu.
With a multicell sampling system it is recommended to measure Zero Cell
spectra in order to correct for slightly different optical properties of the
measurement cells. You should initialize the multicell transport with identical
blank medium in all cells. The Zero Cells… command measures a true blank
spectrum for cell 1, then measures spectra for the remaining cells. The
difference spectra is obtained by subtracting the blank spectrum from the
individual cell spectra are then subtracted automatically from all subsequent
measured spectra to correct for any cell-to-cell optical differences.
1 Fill all measurement cells with the same blank medium (for example,
water).
2 Make sure, that there are no bubbles or floating particles in the cells.
3 Do a Blank measurement by selecting Blank in the measurement menu, by
clicking the Blank Button on the SideBar of the graphical user interface or
by using F4.
4 Start the Zero Cells measurement from the Measure menu.
The Zero cell spectra are displayed in the Zero Cells Spectra window, which
can be activated in the view menu. Make sure that you do not switch the cells
after the zero cell measurement.
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