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Agilent 8453 UV-visible Spectroscopy System
Operator’s Manual
Agilent Technologies
4
Using your Agilent 8453 UV-visible
Spectroscopy System
Starting Your First Measurement Session 56
Starting Your UV-visible Software 58
Measuring Caffeine Absorbance at 273 nm 59
Saving Your Parameters as a Method 62
Retrieving and Printing a Method 64
Saving and Retrieving Data 67
Print Preview of Reports 73
Finding the Caffeine Absorbance Maximum 76
Entering your Cell’s Path Length 80
Controlling your Sipper System 81
Using your Multicell Transport 83
Quantitative Analysis using a Calibration with Standards 86
How Can I Be Sure That My Agilent 8453 Works Properly? 93
How Can I Get a Deeper Understanding of UV-visible Spectroscopy? 96
When Do I Have to Measure a Blank? 98