Understanding Your Agilent ChemStation 125
Quantification
5
ISTD Calculation
ISTD Calculation of Calibrated Peaks
The equations used to calculate the actual amount of a calibrated component x
for a single-level calibration are:
where:
RF
x
is the response factor for compound x;
The actual amount (Actual Amt) of ISTD is the value that was entered in the
Calibration Settings dialog box or the Sample Info dialog box for the internal
standard added to the unknown sample;
M is the multiplier.
D is the dilution factor.
If the ISTD% report type is selected, the following equation is used to calculate
the relative (%) amount of component x:
ISTD Calculation of Uncalibrated Peaks
There are two ways to define the response factor which is used to calculate the
amount for unidentified peaks.
1 Use the fixed response factor set in the With Rsp Factor box of the
Calibration Settings dialog box. You can choose to correct the fixed
response factor by specifying an ISTD correction.
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