24 Biochemical Analysis Software for Agilent ChemStation
2 Kinetics Mode
To measure a single spectrum of the solution in the cell and display it in the
Sample Spectra window select Sample (Single Spectrum) in the measure
menu. You can also measure a single spectrum by pressing F5 or the Sample
Button in the SideBar of the graphical user interface.
Pressing F7, selecting time based measurement from the measure menu or
from the SideBar of the graphical user interface prepares the software for
starting the time based measurement. Now the Time Trace window and the
Sample Spectra window are displayed. Choose Start from the new menu bar to
start the acquisition or Abort to abort an acquisition in progress.
During the Time Based Measurement you can follow the acquisition of the
spectra and the time traces in real-time in the Time Trace window and the
Sample Spectra window. Use the left double-click on the mouse in the Time
Trace window area or the Sample Spectra window area to autoscale the y-axis.
After the Time Based Measurement has been finished or aborted by the user,
the kinetic data are stored as specified in the Options & Info… dialog box. The
Time traces are evaluated by the rate type, which has been defined in the Time
& Calculation dialog box. These results are displayed in the Results window.
Evaluating your Kinetic Data
After the time based measurement has been finished you have the possibility
to reevaluate the kinetic data. Since full spectra have been acquired during the
measurement, the parameters in the fields Wavelength and Rate Calculation of
the Time & Calculation dialog box can be changed. Click on OK to make the
changes active. The new results are displayed in the Results window. If the
method parameter has been changed the modified flag Mod will appear on the
graphical user interface.
If manual sampling system has been configured, you can choose Edit Sample
Information from the Results window to display the Sample Information
dialog box. It allows you to include sample information with your results. The
Sample Information dialog box contains the following parameters:
• The Sample Name.
• A comment (for example a description of the sample)
• The concentration of a substrate in the [S] (mg/ml) field; this field is
present only if you have selected [S] or [S][I] in the Configure Substrate,
Inhibitors dialog box.
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