Biochemical Analysis Software for Agilent ChemStation 5
Contents
1 Installation and Configuration 7
Installing the Biochemical Analysis Software 8
Configuring your Agilent 89090A Peltier Temperature Controller 9
2Kinetics Mode11
Preparing a Time Based Measurement 12
Starting the Kinetics Mode 12
Setting Up your Spectrophotometer 14
Configuring the Sampling System 16
Setting Up a Kinetic Method 18
The Kinetic Measurement 23
Running a Time Based Measurement 23
Evaluating your Kinetic Data 24
Viewing Results 26
3 Thermal Denaturation Mode 27
Preparing a Thermal Denaturation Measurement 28
Starting the Thermal Denaturation Mode 29
Setting Up your Spectrophotometer 30
Setting Up the Temperature Ramp 30
Setting Up a Thermal Denaturation Method 31
The Thermal Denaturation Experiment 36
Running a Thermal Denaturation Experiment 36
Evaluating your Thermal Denaturation Data 37
Viewing Results 38
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