142 Understanding Your Agilent ChemStation
7 Calibration
Definition of Terms
Definition of Terms
Calibration Calibration is the process of determining response factors used to calculate
absolute component concentrations by injecting specially prepared calibration
samples. The calibration table is also used for identification. See “Peak
Identification” on page 127.
Compound A chemical compound can comprise several peaks, in a multiple signal
calibration, typically one per signal. In a single signal calibration a compound
refers to one peak.
Calibration Level A calibration level comprises the calibration points for one calibration sample
concentration. In a multisignal calibration the calibration points can be
distributed over several signals.
Calibration Point A calibration point refers to an amount/response ratio for a peak on the
calibration curve.
Calibration
Sample
A calibration sample, also referred to as a calibration standard or a standard
mixture, is a sample containing a known amount of the compound to quantify.
In the software the calibration sample is referred to as an injection from the
calibration sample vial.
Calibration samples may be purchased from chemical suppliers or they may be
prepared using an accurately measured amount of the pure compound. The
amount of the compound in the calibration sample is usually expressed as a
concentration, typically in ng/µl units.
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