30 Biochemical Analysis Software for Agilent ChemStation
3 Thermal Denaturation Mode
Setting Up your Spectrophotometer
Select the Instrument menu of the biochemical analysis software to setup your
Agilent 8453 spectrophotometer for a thermal denaturation measurement. The
setup of the core spectrophotometer parameters is identical to that in the
kinetics mode of your Agilent ChemStation (see “Setting Up your
Spectrophotometer" on page 14).
Setting Up the Temperature Ramp
In thermal denaturation mode the Peltier temperature controller must be set
as sampling system, which is displayed as Thermostattable Cell in the
sampling field of the instrument panel. Press the Setup button next to the
sampling field to open the Temperature Ramp dialog box, which allows you to
set up the temperature program for the thermal denaturation experiment.
Each line in this dialog box defines a temperature ramp.
• The No. column contains the number of the temperature ramp; temperature
ramps are numbered in sequence.
• The Start column contains the starting temperature for the ramp. When you
append a ramp, the default start temperature is the stop temperature of the
previous ramp.
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