Applied Biosystems QuantStudio 6 Flex User Manual

Table 5 Uniformity calibration troubleshooting
Problem/symptom Possible cause Action
Uniformity calibration failed.
• Plate or array card was
centrifuged insufficiently.
• Plate or array card contains old
or insufficient reagents.
• Abnormally low spectra across
all wells of the plate or array
card.
CAUTION! PHYSIC
AL INJURY HAZARD. During operation, the
sample block can reach 100℃. Before removing the plate or array
card, wait until the block reaches room temperature.
1. Unl
oad the QuantStudio
™
6 or 7 Flex System and confirm that you
loaded the correct plate or array card into the instrument. If not,
perform the calibration again using the correct type of consumable.
2. Unload the QuantStudio
™
6 or 7 Flex System and view the wells of the
plate or array card. If the liquid in the wells of the consumable is not:
• At the bottom of the wells, centrifuge the plate or array card for a
longer time, then repeat the calibration.
• Equivalent in volume, the plate or array card is not sealed and the
reagents have evaporated. Discard the consumable and run
another.
3. If the calibration plate or array card appears to be normal, discard it
and run another.
4. If the calibration fails again, contact Technical Support.
One or more wells produced spectra
that deviate significantly from the
rest of the plate or array card.
1. While viewing the calibration data in the Analysis screen, locate the
well(s) with abnormal signal in the Plate Layout tab.
2. Rotate the plate or array card 180°, then perform the calibration
again.
3. Determine the location of the contaminated wells again. If the
position(s) of the well(s) identified in the steps above are:
• Identical – The sample block is contaminated. Decontaminate the
sample block.
• Reversed – The ROI plate or array card is contaminated. Discard
the consumable, then perform the uniformity calibration using a
new ROI plate or array card.
4. If the calibration fails again, contact Technical Support.
Chapter 2 Calibrate the instrument
Troubleshooting
2
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
75

Applied Biosystems QuantStudio 6 Flex Specifications

General

Brand	Applied Biosystems
Model	QuantStudio 6 Flex
Category	Laboratory Equipment
Language	English