Hitachi 902 - Internal Standard Solution Pipetting + Water + Stirring; ISE Measurement Sequence; Sample Aspiration; Sample Pipetting

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5.4 ISE Measurement Sequence
For the dilution bath in ISE measurement, be sure to use two connected reaction cuvettes.
(1) Sample Pipetting
Cuvette no. n + 2 n + 1 n
Cycle no. 11 12 13
Stop no. 1 1 1
(2) Diluent Pipetting + Water + Stirring
Cuvette no. n + 2 n + 1 n
Cycle no. 11 12 13
Stop no. 3 3 3
(3) Internal Standard Solution Pipetting + Water + Stirring
Cuvette no. n + 2 n + 1 n
Cycle no. 12 13 14
Stop no. 3 3 3
(4) Sample Aspiration
Cuvette no. n + 2 n + 1 n
Cycle no. 17 18 19
Stop no. 1 1 1
S. probe
SMP.
Sample pipetting volume: Fixed at 10 µL
S. probe
DIL
SMP.
Diluent pipetting volume : Fixed at 308 µL
Water : Fixed at 182 µL
Total 490 µL
DIL
SMP.
IS
S. probe
Internal standard
solution pipetting volum : Fixed at 318 µL
Water : Fixed at 182 µL
Total 500 µL
ISE sipper
DIL
SMP.
IS
Sample measurement