3 - 3
3.3 Flow Rate at Each Location
Location
Flow Rate
mL/min
Remarks
S probe Outside
washing
270 ± 30
SV unit restrictor ø1.5/tube L = 80, restrictor ø1.0/tube L = 650
Tube = Tygon ø3.17 × ø6.35
Inside
washing
35 ± 5
R probe Outside
washing
270 ± 30
SV unit restrictor ø1.5/tube L = 80, restrictor ø1.0/tube L =820
Tube = Tygon ø3.17 × ø6.35
Inside
washing
120 ± 20
Stirring and washing
320 ± 30
SV unit restrictor ø1.5/tube L = 80, restrictor ø1.15/tube L =
1000 Tube = Tygon ø3.17 × ø6.35
Rinsing High
concentration
waste solution
probe
90 ± 10
SV11/branch tube/tube L = 395/high
concentration waste solution probe
Tube = Tygon ø2.38 × ø3.96
Low
concentration
waste solution
probe
90 ± 10
Same as above
Cell blank washing
450 ±150
(µL/0.8 sec)
SV unit restrictor ø1.5/tube A = 580, specials/tube B = 500
Tube A = Tygon ø3.17 × ø6.35, tube B = Silastic tube ø1.0 ×
ø3.0
Nozzle tip washing
140 ± 15
SV unit restrictor ø1.5/tube L = 1080
Tube = Tygon ø2.38 × ø3.96
Incubation bath flow
rate
After pouring water into the incubation bath with cells set, flow
velocity on water surface should be measured with cells
removed (in sec/circumference).
Returning from water
supply pump
3.7 ± 0.2
(L/min)
Flow rate through lamp 0.30 or more
(L/min)
Measure with the lamp coolant tube disconnected at the lamp
inlet.
Restrictor ø1.0
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