Hitachi 902 - Page 205

9 - 2
No.
Analytical Method Photometric Point Setting Condition
on Chemistry Parameters Screen
Minimum Necessary
Total Liquid Volume (µµL)
Cell Blank Value
Calculated
Absorbance
Remarks
(9) RATE-
B
(a)
Mode
1
First-half test A l -m -0 -0
3 ≤ l < m < n < p≤35
l + 2 < m
(S + V1) ≥ 180
C C
1 2
2
+ ∆A
l m•
Second-half
test B
n -p -0 -0
3 ≤ l < m < n < p≤35
C C
3 4
2
+
∆A
n p•
When wavelength is
different from that in
first-half test
n + 2 < p
∆ ∆A k A
n p l m• •
−
When wavelength is
different from that in
first-half test
(b)
Mode
2
First-half test A l -m -0 -0
3 ≤ l < m < n < p < q < r≤35
l + 2 < m
(S + V1) ≥ 180
C C
1 2
2
+ ∆A
l m•
Second-half
test B
n -p -q -r
3 ≤ l < m < n < p < q < r≤35
n + 2 < p, p + 2 < q
C C
3 4
2
+
∆ ∆A k A
q r n p• •
−
(Legend)
l, m, n, p, q, r : Photometric points
S : Sample volume
V1, V2, V3 : Each set volume of reagents 1, 2 and 3
V : Total volume of reagents added
C
1
to C
4
: Passed cell blanks
A
x
: Absorbance at photometric point x (*)
∆A
x y•
: Absorbance change rate per minute between photometric points x and y as obtained by least squares method
t : Time (minute) between photometric points l and m
k : Liquid volume compensation factor
k
S V
S V
a
b
=
+
+
V
a
, V
b
: Total volume of reagent added up to photometric point a or b
NOTE* : The absorbance value indicated or output by the instrument is the one obtained by multiplication with 10
4
and
compensation with the cell blank value corresponding to each analytical method.
For calculation, the indicated or output value should be multiplied by 10
-4
.