1220 Infinity II LC System User Manual 137
Detector Description
8
Match the Flow Cell to the Column
Choosing a Flow Cell for the DAD
Figure 49 Choosing a Flow Cell in HPLC
Flow Cell Path Length
Lambert-Beer’s law shows a linear relationship between the flow cell path
length and absorbance.
where
T is the transmission, defined as the quotient of the intensity of the transmitted
light I divided by the intensity of the incident light, I
0
,
e is the extinction coefficient, which is a characteristic of a given substance
under a precisely-defined set of conditions of wavelength, solvent,
temperature and other parameters,
C is the concentration of the absorbing species (usually in g/l or mg/l), and
d is the path length of the cell used for the measurement.
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