Agilent 8453 UV-visible Spectroscopy System Operator’s Manual 11
Introduction to Your System 1
Agilent 8453 Spectrophotometer — Overview
• Lamps
The light source for the UV wavelength range is a deuterium lamp with a
shine-through aperture. As a result of plasma discharge in a low pressure
deuterium gas, the lamp emits light over the 190 nm to approximately
800 nm wavelength range. The light source for the visible and SWNIR
wavelength range is a low-noise tungsten lamp. This lamp emits light over
the 370 nm to 1100 nm wavelength range.
• Source Lens
The source lens receives the light from both lamps and collimates it. The
collimated beam passes through the sample (if one is present) in the sample
compartment.
• Shutter
The shutter is electromechanically actuated. It opens and allows light to
pass through the sample for measurements. Between sample measurements
it closes to limit exposure of sample to light. If the measurement rate is very
fast, you can command the shutter to remain open (Agilent ChemStation
software) or it stays open automatically (handheld controller software).
Figure 1 Optical System of Spectrophotometer
Tun g sten
lamp
Source lens
Deuterium
lamp
Shutter/stray
light filter
Cuvette
Photo diode
array
Source lens
Slit
Grating
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