23
Theory of Operation
Instrument Description
• Green—the instrument is ready to measure.
• Green, blinking—the instrument is measuring.
• Yellow—the instrument is in not-ready state, for example, turning one of
the lamps on or if both lamps are switched off.
• Red—error condition, that is, the spectrophotometer does not pass one of
the self-tests which are run when the spectrophotometer is turned on or an
error occurred during operation. In this case the UV-Visible operating
software gives a detailed error message and possible explanations are in
the online help system and in Chapter 3 “Diagnostics and
Troubleshooting”.
• Red, blinking—error condition of the spectrophotometer processor
system. Because in this case there is no communication with the computer
there will be no error message. The online help system and Chapter 3
“Diagnostics and Troubleshooting” give more information about
troubleshooting.
NOTE When using an Agilent 8453E UV-vis spectroscopy system, you will only have
access to the online help of the handheld controller, when there is no power
loss at the CAN interface of the Agilent 8453 spectrophotometer.
The four measure push buttons on the front panel cause the following actions
to be performed and the resulting data being sent to the computer.
• B
LANK—the instrument takes a blank measurement. This comprises a
reference measurement that is used in all subsequent sample
measurements until a new blank measurement is taken. Following the
reference measurement the baseline spectrum is measured and displayed
on the PC.
• S
AMPLE—the instrument takes a sample measurement or starts a series of
measurements. This depends on the parameters set in your software.
• STANDARD—the instrument takes a measurement of a standard. Additional
information such as concentration and so on, have to be entered in the
operating software.
• S
TOP—the instrument and/or software aborts any ongoing activity and
returns to a to ready state. The stop button is not supported when the
handheld controller of the Agilent 8453E UV-vis spectroscopy system is
used to control the spectrophotometer.
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