EasyManua.ls Logo

Agilent Technologies 8453 User Manual

Agilent Technologies 8453
632 pages
To Next Page IconTo Next Page
To Next Page IconTo Next Page
To Previous Page IconTo Previous Page
To Previous Page IconTo Previous Page
Page #133 background imageLoading...
Page #133 background image
27
Theory of Operation
Instrument Layout and Construction

Table of Contents

Other manuals for Agilent Technologies 8453

Question and Answer IconNeed help?

Do you have a question about the Agilent Technologies 8453 and is the answer not in the manual?

Agilent Technologies 8453 Specifications

General IconGeneral
TypeUV-Visible Spectrophotometer
Wavelength Range190 - 1100 nm
Spectral Bandwidth1 nm
Wavelength Accuracy±0.5 nm
Data Interval1 nm
Power Requirements100 - 240 VAC, 50/60 Hz
Weight15 kg
Light SourceDeuterium and Tungsten Halogen lamps
Photometric Noise0.0002 Abs
Stray Light< 0.05% at 220 nm
InterfaceRS-232
SoftwareAgilent ChemStation
Photometric Accuracy± 0.005 AU at 1 AU (verified with NIST traceable standards)

Summary

Notices

Safety Notices

Important warnings and cautions for safe operation and to prevent product damage or data loss.

Installation and Start Up

Installation Summary

Outlines key steps for installing the spectrophotometer and associated PC components.

Starting a Measurement Session

Provides step-by-step instructions to initiate the system and begin a measurement.

Good Measurement Practices

Using the Agilent 8453 Spectroscopy System

Starting Your First Measurement Session

Step-by-step instructions for system startup and performing the initial measurement.

Quantitative Analysis with Calibration Standards

Guide to performing quantitative analysis using calibration standards and methods.

Verifying Agilent 8453 Instrument Functionality

Instructions on performing quick checks and self-tests to confirm instrument operation.

Instrument Specifications

Performance Specifications

Lists technical performance metrics like wavelength range, resolution, accuracy, and noise.

Diagnostics and Troubleshooting

Front Panel Status and Power Switch LEDs

Describes typical LED indicators and their meaning in case of instrument failures.

Error Messages and Troubleshooting

Lists error messages, their causes, and suggested actions for resolution.

General Troubleshooting Hints

Practical tips for diagnosing and resolving instrument problems not directly indicated by error messages.

Maintenance and Repair Procedures

Instrument Maintenance

General maintenance tasks including cleaning the instrument and lamps.

Repair Procedures

Detailed steps for performing instrument repairs, including handling electronic assemblies safely.

Exchanging Deuterium or Tungsten Lamps

Step-by-step guide for replacing the instrument's deuterium and tungsten lamps.

Interfacing and Communication

Setting the 8-Bit Configuration Switch

Explains how to configure instrument settings using the 8-bit configuration switch.

Agilent ChemStation Features

Data Acquisition Process

Explains the workflow for acquiring data for sequences and single runs.

Data Analysis Integration

Explains the ChemStation integrator algorithms and their capabilities.

Data Analysis Quantification

Details the calculation procedures for determining component concentrations.

Data Review, Reprocessing, and Batch Review

Covers reviewing and reprocessing sequence data, and concepts of batch review.

System Suitability Reports

Enables reporting of system performance parameters for individual analyses.

Instrument Control

Explains how ChemStation software controls instruments and enables mixed technique configurations.

Methods

Data Acquisition

Peak Integration

What is Peak Integration?

Defines integration as the process of locating peaks and calculating their sizes for analysis.

What Integration Does

Describes how the software identifies peak start/end times, apex, and calculates area/height/width.

ChemStation Integrator Algorithms

Details the capabilities and algorithms used by the ChemStation integrator.

Baseline Allocation Methods

Explains how the integrator allocates baselines using various techniques and parameters.

Quantification Procedures

Quantification Calculation Methods

Details calculation procedures like Percent, Normalization, ESTD, and ISTD.

Peak Identification

Calibration Procedures

Recalibration Process

Describes updating calibration levels with new sample data to maintain analysis accuracy.

Automation Features

Data Review, Reprocessing, and Batch Review

What is Batch Review?

Describes batch review as a capability for performing a first-pass review of sequence results.

Evaluating System Suitability

Noise Determination Methods

Describes methods for calculating noise from signal data points within a selected time range.

System Verification

Performing System Verification

How to verify ChemStation software operation by reprocessing data files against standards.

Related product manuals