Agilent 8453 UV-visible Spectroscopy System Operator’s Manual 15
Introduction to Your System 1
Agilent 8453 Spectrophotometer — Overview
On the front panel of the spectrophotometer is a status indicator which will
display different colors depending of the actual condition of the
spectrophotometer.
• Green—the spectrophotometer is ready to measure.
• Green, blinking—the spectrophotometer is measuring.
• Yellow—the spectrophotometer is in not-ready state, for example, turning
one of the lamps on or if both lamps are switched off.
• Red—error condition, that is, the spectrophotometer does not pass one of
the self-tests which are run when the spectrophotometer is turned on or an
error occurred during operation. In this case the UV-visible operating
software gives a detailed error message and possible explanations are in
the online help system and in your Service Manual Chapter 3 “Diagnostics
and Troubleshooting”.
• Red, blinking—error condition of the spectrophotometer processor system.
Because in this case there is no communication with the computer there
will be no error message. The online help system and your Service Manual
Chapter 3 “Diagnostics and Troubleshooting” give more information about
troubleshooting.
The four measure push buttons on the front panel cause the following actions
to be performed and the resulting data being sent to the computer. The push
button functionality is controller by the UV-visible ChemStation software and
adjusted to the current measurement task.
• B
LANK—the spectrophotometer takes a blank measurement. This comprises
a reference measurement that is used in all subsequent sample
measurements until a new blank measurement is taken. Following the
reference measurement an additional baseline spectrum is measured and
displayed on the PC.
• S
AMPLE—the spectrophotometer takes a sample measurement or starts a
series of measurements. This depends on the parameters set in your
software.
• S
TANDARD—the spectrophotometer takes a measurement of a standard.
Additional information such as concentration and so on, have to be entered
in the operating software.
• S
TOP—the spectrophotometer and/or software aborts any ongoing activity
and returns to a to ready state.
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