Agilent 8453 UV-visible Spectroscopy System Operator’s Manual 53
Good Measurement Practices 3
Inserting a Cell
Inserting a Cell
Your spectrophotometer is shipped with the standard single-cell holder you
first have to install in the sample compartment. This cell holder
accommodates standard cells or flow cells. To insert a sample cell in the cell
holder:
1 Move the locking lever to its up position. 2 Insert the sample cell, making sure you orient it
correctly. The frosted (non-clear) sides of the
sample cell should not be in the path of the light
beam.
3 Lock the sample cell in place by pushing the locking
lever back down.
Utmost care should be taken when using small
volume flow cells and particularly any cells with less
than a 2 mm aperture. Here is is imortant that the
cells are properly centered in the light path. The cell
holder is designed for cells with 15 mm center
height. If applicable these cells should not be
removed between the respective reference (blank)
measurement and the sample measurement.
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