ChemStation Workflow 15
Data Acquisition
2
Data Acquisition
available for future data review and reprocessing, without changes that
were applied to the master method or sequence template for other
sequence runs.
During acquisition, the data files are stored to the sequence container.
Within each data file (*.D), two additional methods, ACQ.M and DA.M, are
saved for this specific run. These two methods are copies of the sequence
method, preserving the state of the method as it was at the time of
acquisition of the specific data file. In the case of e.g. calibration table
updates the DA.M methods differ for each of the runs.
The individual acquisition method ACQ.M is intended to preserve the
acquisition parameters, therefore it is recommended that you do not
change this method during future data review activities. In Data Analysis
view, the acquisition parameters of this method can be viewed and
printed.
With these files saved in the sequence folder, all data review and
reprocess activities can be performed without altering the master method
or the sequence template. If needed, method changes can also be saved to
the master method again.
Partial Sequence Acquisition
In the case of partial sequence acquisition, the user can decide between
two options:
• acquire the partial sequence into a new sequence container
or
• acquire the partial sequence in an already existing sequence container.
Acquiring the data files from a partial sequence execution into an already
existing sequence container may be useful in the following scenarios:
• A single data file (or several data files) have to be overwritten, because
e.g. an incorrect vial was used in the first place.
• Only the first part of the sequence has been executed in the first place,
and the missing samples have to be added by executing partial
sequence. This may occur in the case of an instrument failure during
sequence acquisition.
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