6 Agilent 8453 UV-visible Spectroscopy System Operator’s Manual
Contents
3 Good Measurement Practices 39
General Considerations 40
Spectrophotometer Design 40
Making Measurements 40
Sample Cell Material 41
Optical Specifications of Cells 42
Apertured Cells 43
Flow Cells 44
Handling and Maintaining Cells 45
Solvents 47
Sample Preparation 48
Photosensitive Samples 49
Stirring and Temperature Control 50
Checklist for Best Results 50
Inserting a Cell 53
4 Using your Agilent 8453 UV-visible Spectroscopy System 55
Starting Your First Measurement Session 56
Starting Your UV-visible Software 58
Measuring Caffeine Absorbance at 273 nm 59
Saving Your Parameters as a Method 62
Retrieving and Printing a Method 64
Saving and Retrieving Data 67
Saving your Samples 67
Saving a Selected Spectrum 69
Retrieving Spectra 71
Deleting Current Spectra 72
Print Preview of Reports 73
Finding the Caffeine Absorbance Maximum 76