Biochemical Analysis Software for Agilent ChemStation 33
Thermal Denaturation Mode 3
• The value in the Filter Length field sets the number of data points that is
used to calculate each point in the derivative trace. The value must be an
odd number between 5 and 749; the default value is 21. Increasing the
number of points increases the degree of smoothing of the resulting trace.
• Only maxima and minima that are at least the value in the Sensitivity field
distinct from their neighboring maxima or minima are reported. When the
field is left blank, the sensitivity is set to 1/1000 of the difference between
the maximum and minimum values.
The Equation group allows you to define the equation for the calculation of a
result.
• Type a parameter name for the result in the field to the left of the equation
and the equation in the field to the right. Use Tm (melting temperature), M
(molarity of the sample), and K (DNA length).
• The Equation for Thermal Expansion group allows you to select to make a
correction for the change in absorbance due to the thermal expansion of the
solution on heating. Select Volume Correction to specify that a correction
for thermal expansion should be made. Type the equation for the volume
correction in the Volume (T) = field. You can use the same operators in the
volume correction equation as in the Equation group. Use the variable T for
the sample temperature.
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