Agilent 8453 UV-visible Spectroscopy System Operator’s Manual 77
Using your Agilent 8453 UV-visible Spectroscopy System 4
Finding the Caffeine Absorbance Maximum
4 Type 2 for the number of peaks to find and uncheck the valley find option.
Set your data type to absorbance and adjust your spectral display to a
wavelength range from 190 nm to 400 nm in the dialog’s Display spectrum
section. Click OK to set your parameters.
5 Fill your 1-cm path-length quartz cell with distilled water. Lift the lever at
the left side of your cell holder. Put the cell in the cell holder and make sure
the transparent windows face towards the font and the back of the
spectrophotometer. Push the lever down to secure your cell in the cell
holder.
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