178 1220 Infinity II LC System User Manual
9
Test Functions and Calibration
Diode Array Detector (DAD)
• does the UV-lamp show a reflective coating towards the VIS-lamp? If yes,
replace UV-lamp.
Example (measured without flow cell):
Test Failed
VIS-LAMP OFF or defect: 110 counts
VIS-LAMP ON and OK: 13613 counts
Probable cause Suggested actions
1
Absorbing solvent or air bubble in flow cell.
Ensure the flow cell is filled with water, and
free from air bubbles.
2
Dirty or contaminated flow cell.
Run the cell test (see “Cell Test” on page 184).
If the test fails, exchange the flow cell
windows.
3
Dirty or contaminated optical components
(achromat, windows).
Clean optical components with alcohol and
lint-free cloth or replace the parts.
4
Old or non-Agilent lamp.
Exchange the lamp.
If the lamp fails in a single range there might be no reason to change the lamp if the
application is not run in that specific range.
Redo the test with removed flow cell. If the counts increase drastically (more than a factor
of 2, then flow cell components are contaminated and may require maintenance/service.
If the intervals of lamp replacements are getting shorter, the Agilent service should check
the optical unit for contaminated components in the light path (coupling lens, source lens,
cell support assembly and flow cell windows).
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