1220 Infinity II LC System User Manual 179
Test Functions and Calibration
9
Diode Array Detector (DAD)
Holmium Oxide Test
The holmium oxide test uses characteristic absorbance maxima of the built-in
holmium oxide filter to verify wavelength accuracy (see also “Wavelength
Verification and Recalibration” on page 188). When the test is started, the
1-nm slit is moved into the light path automatically. To eliminate effects due to
absorbing solvents, the test should be done with water in the flow cell or with
removed flow cell.
Holmium Oxide Test Evaluation
Limits:
The test is evaluated by the instrument, and the measured maxima are
displayed automatically. The test fails if one or more of the maxima lies
outside of the limits (see Figure 55 on page 181).
See also “Declaration of Conformity for HOX2 Filter” on page 389.
361.0 nm 360.0 - 362.0 nm (± 1nm)
453.7 nm 452.7 - 454.7 nm (± 1nm)
536.7 nm 535.7 - 537.7 nm (± 1nm)
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