Agilent 8453 UV-visible Spectroscopy System Operator’s Manual 57
Using your Agilent 8453 UV-visible Spectroscopy System 4
Starting Your First Measurement Session
6 The first task you have to perform is to measure a reference. Typically the
cell containing the solvent used with your samples is put in the
measurement position and a blank measurement performed. To start this
measurement, click the Blank button on your Instrument Panel or press the
spectrophotometer’s Blank button.
A blank measurement is a reference measurement combined with the
measurement of a baseline spectrum. A baseline spectrum gives you
additional hints on the absorbance of the cell windows and the solvent.
Areas with high noise indirectly indicate high absorbance.
7 The next measurement is your sample measurement. To get the most
precise results, use the same cell in the same orientation to the
measurement beam. Flush your cell about three times with your sample
solution and start the measurement by clicking the Instrument Panel’s
Sample button or by pressing the spectrophotometer’s Sample button.
NOTE
For high precision measurements, wait until the spectrophotometer and the lamps have
reached thermal equilibrium. The time required is a function of the environmental
conditions. Your spectrophotometer should be ready after 45 minutes.
NOTE
For details on how to mount your cell, see “Inserting a Cell" on page 53.
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