60 Agilent 8453 UV-visible Spectroscopy System Operator’s Manual
4 Using your Agilent 8453 UV-visible Spectroscopy System
Measuring Caffeine Absorbance at 273 nm
4 Type the wavelength of interest in the Wavelengths section of the Fixed
Wavelength(s) Parameters dialog. Adjust your spectral display to a
wavelength range from 190 nm to 400 nm in the Display spectrum section.
Click OK to set your parameters.
5 Fill your 1 cm path length quartz cell with distilled water. Lift the lever at
the left side of your cell holder. Put the cell in the cell holder and make sure
the transparent windows face towards the font and the back of the
spectrophotometer. Push the lever down to secure your cell in the cell
holder.
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